Innovative Mechanical Processing of Adipose Tissue: A Comparative Evaluation of Microfat and Concentrated Micronized Fat for Superficial Fat Grafting.

Early human evidence such as case series or small samples is exploring possible benefits.

• Level A · Stronger Clinical Evidence
• Level B · Emerging clinical evidence with positive signals
• Level C · Early human research exploring benefits
• Level D · Scientific groundwork from lab and animal studies
• Emerging · Emerging topic under active research

Abstract (original English)

Background Superficial autologous fat grafting is widely used for facial rejuvenation and regenerative treatments, yet its retention rate remains unpredictable. Enhancing the concentration of adipose-derived stem cells (ASCs) has been shown to improve graft retention. Mechanical fat processing techniques can reduce fat granules size for superficial injection as well as elevate the concentration of ASCs. Objectives We introduced a novel mechanical fat processing method to convert macrofat into concentrated micronized fat (CMF), which can generate small fat particles and increase the concentration of ASCs. The microstructure, cell composition, and in vivo evaluation of microfat and CMF were compared. Methods Adipose tissue was harvested from patients undergoing liposuction. Microfat was obtained using a multiperforated cannula with multiple 1.0-mm smooth side holes, while CMF was prepared with a spinning sharp blade to create smaller particles. Cell counting, viability assays, flow cytometry, ELISA, and in vivo studies were conducted to evaluate the processed fat. Results Both CMF and microfat were suitable for superficial fat grafting with 26-gauge needles. Scanning electron microscopy and whole-mounting stain showed that the CMF maintained an integral histological structure. Cell count and flow cytometry tests revealed that the number of ASCs increased significantly in the CMF

Evidence level

Early human evidence such as case series or small samples is exploring possible benefits.

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