Insights Into Dose-Dependent Bone Toxicity Following Partial-Body Exposure to Fractionated Ionizing Radiation In Vivo.

Evidence from laboratory and animal studies provides groundwork for understanding mechanisms and potential before human studies continue.

• Level A · Stronger Clinical Evidence
• Level B · Emerging clinical evidence with positive signals
• Level C · Early human research exploring benefits
• Level D · Scientific groundwork from lab and animal studies
• Emerging · Emerging topic under active research

Abstract (original English)

Bone toxicity resulting from ionizing radiation (IR) is a major contributor to bone loss, fracture, pain, and morbidity. However, the precise mechanisms that drive its development remain largely unknown, and no effective medical countermeasure exists. Deciphering the molecular mechanism and mode of action as well as discerning molecular biomarker signatures that manifest proportionate to the dose and severity of injury is crucial in expediting the discovery and development of novel and effective diagnostic, prognostic, and treatment approaches. Herein, we investigate and compare the response to high dose IR during the transition of bone-derived mesenchymal stem cells to osteoblasts and adipocytes, macrophages to osteoclasts, and late osteoblasts to mature osteocytes in vitro. Further, the counter response of bone to cumulative doses of 8, 16, and 24 Gy was assessed in vivo. Our findings indicate several novelties: cumulative radioresistance to DNA damage, apoptosis, reactive oxygen species formation, and dysfunctional mineral deposition was measured during osteoblast to osteocyte transition. Irradiation stimulated the formation of tunneling nanotube-like structures, a novel type of intercellular communication machinery, in exposed macrophages. Bone fracture stress but not ultimate stress significantly decreased 48 h after a single 8 Gy exposure and prior to microarchitectural d

Evidence level

Evidence from laboratory and animal studies provides groundwork for understanding mechanisms and potential before human studies continue.

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