Mechanical Processing of Lipoaspirate With a Fluidic Device Platform Promotes Wound Healing Transcriptional Programs and Angiogenesis In Vitro.

Evidence from laboratory and animal studies provides groundwork for understanding mechanisms and potential before human studies continue.

• Level A · Stronger Clinical Evidence
• Level B · Emerging clinical evidence with positive signals
• Level C · Early human research exploring benefits
• Level D · Scientific groundwork from lab and animal studies
• Emerging · Emerging topic under active research

Abstract (original English)

Background Mechanical processing of lipoaspirate (LA) produces a stromal vascular fraction (SVF) without enzymatic digestion for use in aesthetic, surgical, and regenerative applications. We recently presented novel device technologies that increased mesenchymal stem cell (MSC) content relative to standard nanofat (NF) processing. Objectives Here, we introduce a third technology designed to enhance fluid shear forces and explore the impact of mechanical processing on regenerative potential in vitro. Methods Human LA samples were processed with our previously reported emulsification micronization device and filtration device, and then optimized using a new shearing device (SD). Results were analyzed for total cell count, viability, and percentages of endothelial progenitor cells (EPCs) and MSCs compared to manual NF processing, both immediately and following 24-hour culture. Expression of genes related to wound healing was quantified by real-time quantitative polymerase chain reaction, and angiogenic capacity was determined with an in vitro 3-dimensional sprouting assay. Results The SD did not significantly affect MSC recovery or viability, but EPCs were enriched in a shear stress-dependent manner. Gene expression was not altered immediately after processing, but after culture we noted changes to wound-healing transcriptional programs that were consistently stronger for our devi

Evidence level

Evidence from laboratory and animal studies provides groundwork for understanding mechanisms and potential before human studies continue.

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