METTL3-modified exosomes from adipose-derived stem cells enhance the proliferation and migration of dermal fibroblasts by mediating m6A modification of CCNB1 mRNA.

Evidence from laboratory and animal studies provides groundwork for understanding mechanisms and potential before human studies continue.

• Level A · Stronger Clinical Evidence
• Level B · Emerging clinical evidence with positive signals
• Level C · Early human research exploring benefits
• Level D · Scientific groundwork from lab and animal studies
• Emerging · Emerging topic under active research

Abstract (original English)

Skin scalded injury is a devastating condition. Exosomes derived from adipose-derived mesenchymal stem cells (ASC-exos) have been shown encouraging therapeutic potential in wound healing. Here, we explored the activity and mechanism of methyltransferase-like 3 (METTL3)-modified ASC-exos in the migration and proliferation of dermal fibroblasts. ASC-exos were isolated from mouse ASCs, characterized, and used to incubate mouse dermal fibroblasts. Fluorescence microscopy was used to analyze the transfer of ASC-exos into fibroblasts. Cell migration, invasion, proliferation, and viability were assessed by wound healing, transwell, 5-Ethynyl-2'-deoxyuridine (EdU), and Cell Counting Kit-8 (CCK-8) assays, respectively. Protein expression was tested by western blotting. The influence of METTL3 in cyclin B1 (CCNB1) was evaluated by methylated RNA immunoprecipitation (MeRIP), actinomycin D treatment and quantitative PCR assays. ASC-exos significantly increased the proliferative, invasive, and migratory potentials of dermal fibroblasts. Overexpression of METTL3 resulted in elevated proliferation, invasiveness, and migratory capacity in dermal fibroblasts. Furthermore, METTL3-modified ASC-exos derived from METTL3-increased ASCs exerted more significantly promoting effects on fibroblast proliferation and migration than ASC-exos. Mechanistically, METTL3 upregulated CCNB1 by affecting its mRNA

Evidence level

Evidence from laboratory and animal studies provides groundwork for understanding mechanisms and potential before human studies continue.

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