SVF
Level C· Early human research exploring benefitsCase Report / SeriesPubMed

Molecular Characterization of Lipoaspirates Used in Regenerative Head and Neck Surgery.

Sharaf K., Kleinsasser A., Schwenk-Zieger S., Gires O., Schinke H., Kohlbauer V.

Case Report / Series with a reported sample of 15 on Cartilage Damage, Chronic Wound, published in JAMA Facial Plast Surg (2019) — summary generated from the PubMed abstract.

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Level C· Early human research exploring benefitsEvidence level of this study

Early human evidence such as case series or small samples is exploring possible benefits.

  • Level A · Stronger Clinical Evidence
  • Level B · Emerging clinical evidence with positive signals
  • Level C · Early human research exploring benefits
  • Level D · Scientific groundwork from lab and animal studies
  • Emerging · Emerging topic under active research
Read the A–D evidence level guide

This page is generated from the PubMed record. The Thai description is an automated summary of bibliographic fields and the abstract, not a full translation, and is not medical advice.

Study type
Case Report / Series
Journal
JAMA Facial Plast Surg (2019)
Country
United States
Reported sample size
15
Source database
PubMed
PMID
31556908
PMCID
PMC6764019
DOI
10.1001/jamafacial.2019.0851
Citations
5

Abstract (original English)

Importance Adipose-derived mesenchymal stem cells (ASCs) have been used commonly in regenerative medicine and increasingly for head and neck surgical procedures. Lipoaspiration with centrifugation is purported to be a mild method for the extraction of ASCs used for autologous transplants to restore tissue defects or induce wound healing. The content of ASCs, their paracrine potential, and cellular potential in wound healing have not been explored for this method to our knowledge. Objective To evaluate the characteristics of lipoaspirates used in reconstructive head and neck surgical procedures with respect to wound healing. Design, setting, and participants This case series study included 15 patients who received autologous fat injections in the head and neck during surgical procedures at a tertiary referral center. The study was performed from October 2017 to November 2018, and data were analyzed from October 2017 to February 2019. Main outcomes and measures Excessive material of lipoaspirates from subcutaneous abdominal fatty tissue was examined. Cellular composition was analyzed using immunohistochemistry (IHC) and flow cytometry, and functionality was assessed through adipose, osteous, and chondral differentiation in vitro. Supernatants were tested for paracrine ASC functions in fibroblast wound-healing assays. Enzyme-linked immunosorbent assay measurement of tumor necrosis

What this study does not prove

  • • This study does not prove SVF is an approved treatment or a replacement for standard care.
  • • Without an adequate control group, treatment effects cannot be separated from other factors.

Evidence level

Early human evidence such as case series or small samples is exploring possible benefits.

How we grade evidence
Abdominal FatCell DifferentiationCells, CulturedDysphoniaEnzyme-Linked Immunosorbent AssayFemaleHead and Neck NeoplasmsHumansImmunohistochemistryImmunophenotyping
Cartilage DamageChronic Wound
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