Morphologic changes in photodamaged organotypic human skin culture after treatment of autologous adipose-derived stromal cells.

Several human studies show positive signals, while research methods and sample sizes continue to develop.

• Level A · Stronger Clinical Evidence
• Level B · Emerging clinical evidence with positive signals
• Level C · Early human research exploring benefits
• Level D · Scientific groundwork from lab and animal studies
• Emerging · Emerging topic under active research

Abstract (original English)

Background Recent clinical trials indicate that human adipose-derived stromal cells (hASCs) have beneficial effects on antiaging and wound healing. This study examined the morphologic changes in photodamaged human organotypic skin culture after treatment of autologous hASCs. Methods Abdominal skin flaps were obtained from 8 white females who underwent abdominoplasties with liposuction. The adipose layer was removed and used for hASC isolation. Sections of skin were removed and cultured in serum-free medium. To induce photodamage, some of the skin pieces were irradiated with maximum subcytotoxic doses of UVB (1600 J/m(2)) and UVA (250 J/m(2)). The effects of hASC on skin segments were evaluated by coculture as a feeder layer or by injecting intradermally. Portions of the skin samples were removed for analysis on days 3, 5, 7, and 9 of culture and analyzed histologically for morphology, viability, and proliferation status. Results Epidermal necrosis of irradiated skin was significantly reduced by the presence of hASCs. Increased parakeratosis was observed at early time points, and apoptosis in epidermis was markedly decreased by hASCs. Differences were observed in epidermal differentiation but not basal cell proliferation. Similar results were obtained by both methods of hASC treatment to the skin. Conclusions Exposure of UV-irradiated skin to hASC attenuated cell senescence and

Evidence level

Several human studies show positive signals, while research methods and sample sizes continue to develop.

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