Phenotypic and Functional Characterization of Bovine Adipose-Derived Mesenchymal Stromal Cells.

Evidence from laboratory and animal studies provides groundwork for understanding mechanisms and potential before human studies continue.

• Level A · Stronger Clinical Evidence
• Level B · Emerging clinical evidence with positive signals
• Level C · Early human research exploring benefits
• Level D · Scientific groundwork from lab and animal studies
• Emerging · Emerging topic under active research

Abstract (original English)

Objective: Mesenchymal stem cells (MSCs) are increasingly trialed in cellular therapy applications in humans to treat several degenerative and inflammatory disorders. Not only are MSCs useful as a therapeutic for human diseases, but they can also be applied to treat a range of diseases in animals. Particularly, larger animals such as cattle can benefit from MSC therapies to combat inflammatory conditions and aging-associated degenerative disorders. Given that MSCs have multilineage differentiation and immunomodulatory properties, we sought to demonstrate the feasibility of obtaining MSCs from adipose tissue and characterizing cells using established phenotypic and functional assays. Animals: Adipose tissue from three individual healthy cows was obtained from the tail head area and processed for MSC isolation. Procedures: MSCs were isolated using in-house optimized tissue digestion protocols and characterized by performing colony formation assay, cell growth assessments, cell-surface marker analysis by immunocytochemistry, osteogenic and adipogenic differentiation, and secretion of indoleamine 2,3-dioxygenase (IDO). Results: MSCs were successfully isolated from bovine adipose (bv-Ad) tissue and demonstrated the ability for prolonged culture expansion, colony formation, and differentiation into osteogenic and adipogenic lineages. Bv-AdMSCs secreted significant amounts of IDO with

Evidence level

Evidence from laboratory and animal studies provides groundwork for understanding mechanisms and potential before human studies continue.

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