Phenylboronic acid modified polyethyleneimine alginate chitosan dynamic hydrogels combination with adipose derived stem cells for controlled delivery of BMP2 to promote bone regeneration and modulating macrophage and ang

Evidence from laboratory and animal studies provides groundwork for understanding mechanisms and potential before human studies continue.

• Level A · Stronger Clinical Evidence
• Level B · Emerging clinical evidence with positive signals
• Level C · Early human research exploring benefits
• Level D · Scientific groundwork from lab and animal studies
• Emerging · Emerging topic under active research

Abstract (original English)

Bone morphogenetic protein-2 (BMP2) has been approved by the United States Food and Drug Administration (FDA) to be used in human lumbar fusion to promote bone regeneration. However, BMP2 has a short half-life, rapid degradation, and burst release at high concentrations, which aggravates its adverse effects. A sodium alginate chitosan PEI-PBA-BMP2-ADSCs (SCPPBA) hydrogel was constructed. Hydrogel structure, biocompatibility, osteoinductivity, immunoregulation, and angiogenesis were evaluated. The SCPPBA hydrogel exhibited a porous structure, chemical crosslinking, moderate elastic modulus and viscosity, biocompatibility, biodegradation, and controlled and long-period BMP2 protein release. In an in vitro study, SCPPBA hydrogel exhibited osteoinductivity and immune modulation. We further implanted the SCPPBA hydrogel into a calvarial bone defect model in mice. The SCPPBA hydrogel exhibited excellent bone regeneration capacity and modulated macrophage polarization and angiogenesis in vivo. The SCPPBA hydrogel realizes the long-term controlled delivery of BMP2 and is suitable for BMP2 loaded implantation to promote bone regeneration.

Evidence level

Evidence from laboratory and animal studies provides groundwork for understanding mechanisms and potential before human studies continue.

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