Plasma-rich fibrin gel and adipose-derived allogeneic mesenchymal stem cells: innovation in the treatment of second-degree deep burn wound; characterization and in-vivo study.

Evidence from laboratory and animal studies provides groundwork for understanding mechanisms and potential before human studies continue.

• Level A · Stronger Clinical Evidence
• Level B · Emerging clinical evidence with positive signals
• Level C · Early human research exploring benefits
• Level D · Scientific groundwork from lab and animal studies
• Emerging · Emerging topic under active research

Abstract (original English)

A biocompatible and readily available wound dressing for emergencies has been shown to be more cost-effective, while also reducing the risk of immune system-mediated reactions. In this project, we investigated the use of a fresh blood-derived matrix as a wound dressing, based on a 3D drug-loaded Plasma-rich Fibrin (PRF) scaffold, to support the transplantation of autologous stem cells for regenerating skin tissues lost due to burns. PRF scaffold was prepared from venous blood, and adipose tissue-derived stem cells (ADSCs) were isolated from the visceral fat tissue of rats. Following in vitro analysis, PRF gel and ADSCs were transplanted onto second-degree deep burn wounds on the backs of rats. Histopathological analysis and wound size measurements were conducted on days 5, 10, 15, and 21. The findings revealed that PRF gel, as a cyto-compatible scaffold with the potential for antibacterial drug release (sustained for up to 3 days, with up to 89.7% release), significantly enhanced the healing process in the treatment group. On day 15, a reduced wound size, mature skin cells, and well-organized, thicker collagen fibers were observed in the histopathology of the PRF-treated groups, which scored an average of (2.83 ± 0.04) out of 3 for overall histopathological parameters. The greatest wound contraction was seen in the scaffold-treated groups (5.32 ± 0.61 mm 2 ), compared with the

Evidence level

Evidence from laboratory and animal studies provides groundwork for understanding mechanisms and potential before human studies continue.

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