SVF
Level C· Early Human ResearchProspective Study

Proliferation and Migration of Oral Fibroblasts Activated by Stromal Vascular Fraction-derived Cells: An In Vitro Study.

Keerativittayanun S., Panich I., Thuaksuban N., Rotpenpian N.

Prospective Study with a reported sample of 6 on Chronic Wound, published in Int Dent J (2026) — summary generated from the PubMed abstract.

This page is generated from the PubMed record. The Thai description is an automated summary of bibliographic fields and the abstract, not a full translation, and is not medical advice.

Study type
Prospective Study
Journal
Int Dent J (2026)
Country
England
Reported sample size
6
PMID
41330122
DOI
10.1016/j.identj.2025.109301

Abstract (original English)

Oral wound healing is a complex process that presents significant challenges in patient management. This study aimed to isolate and characterise stromal vascular fraction-derived cells (SVFs) from subcutaneous adipose tissue and investigate the effects of SVF-conditioned media (SVF-CM) on oral fibroblast proliferation and migration. Subcutaneous adipose tissue was collected from 6 patients (aged 9-18 years) during iliac bone graft procedures. SVFs were evaluated for morphology, colony-forming ability, osteogenic differentiation and surface markers. Flow cytometry (passages 2) assessed MSC-specific markers. SVF-CM was collected from passage 2 SVFs and diluted to 0.1X, 0.5X, and 1X concentrations. Oral fibroblast proliferation, viability and migration were evaluated using cell counting, Live/Dead staining and scratch assays for 7 days. SVFs were successfully isolated from 33.33% of samples, showing spindle-shaped morphology and colony formation by day 14. Alizarin Red staining confirmed osteogenic differentiation. Flow cytometry showed partial expression of CD73, CD90, CD105 and negative expression of CD45, CD34 and HLA-DR. SVF-CM significantly enhanced oral fibroblast proliferation and migration in a dose-dependent manner. Live/Dead staining confirmed non-toxicity. SVF-CM promotes oral fibroblast proliferation and migration, suggesting its potential as a therapeutic agent for en

What this study does not prove

  • • This study does not prove SVF is an approved treatment or a replacement for standard care.

Evidence level

Early human evidence such as case series or small samples, often without a control group.

How we grade evidence
HumansCell ProliferationCell MovementFibroblastsAdolescentChildOsteogenesisCell DifferentiationCulture Media, ConditionedIn Vitro Techniques
Face & SkinChronic Wound
View on PubMedDOI

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