Proteome-wide analysis by liquid chromatography tandem mass spectrometry reveals the role of retinoic acid during adipogenesis in human bone mesenchymal stem cells

Evidence from laboratory and animal studies provides groundwork for understanding mechanisms and potential before human studies continue.

• Level A · Stronger Clinical Evidence
• Level B · Emerging clinical evidence with positive signals
• Level C · Early human research exploring benefits
• Level D · Scientific groundwork from lab and animal studies
• Emerging · Emerging topic under active research

Abstract (original English)

Objective Retinoic acid (RA), an active metabolite of vitamin A, may regulate adipogenesis and is associated with osteoporosis. To clarify the regulatory mechanism of RA in adipogenesis and its relationship with the occurrence and development of osteoporosis, we investigated the role of all-trans retinoic acid (ATRA) in protein expression profiling during human bone mesenchymal stem cells (hBMSCs) adipogenesis. Methods Liquid chromatography tandem mass spectrometry (LC-MS/MS) was used to determine the protein profile, and raw data were analyzed against the UniProt database using MaxQuant with the Andromeda search engine. The Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) databases were used for functional annotation of differentially expressed proteins (DEPs). The interaction relationships of DEPs were assessed using the STRING database, and Cytoscape was used to visualize the protein interaction network. Results A total of 5,611 proteins were identified by LC-MS/MS in 15 samples, of which 5,470 proteins showed quantifiable data. When treated with ATRA for seven and 14 days after adipogenic induction, 470 and 1,408 DEPs were upregulated and 508 and 1,345 DEPs were downregulated. Gene functional annotation of DEPs showed that ATRA upregulated classic signaling pathways, such as Wnt, Hippo, and MAPK, as well as cytoskeleton related pathways, including focal

Evidence level

Evidence from laboratory and animal studies provides groundwork for understanding mechanisms and potential before human studies continue.

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