Level D· Preclinical EvidenceAnimal Study

[Proteomics analysis of the effect and mechanism of ADSCs on full-thickness skin defects in diabetic rats].

Gu Y., Chen YZ., Wang SY., Song HY., Bai N.

Animal Study on Type 2 Diabetes, Chronic Wound, published in Zhonghua Shao Shang Yu Chuang Mian Xiu Fu Za Zhi (2026) — summary generated from the PubMed abstract.

This page is generated from the PubMed record. The Thai description is an automated summary of bibliographic fields and the abstract, not a full translation, and is not medical advice.

Study type: Animal Study
Journal: Zhonghua Shao Shang Yu Chuang Mian Xiu Fu Za Zhi (2026)
Country: China
Reported sample size: —
PMID: 41611293
DOI: 10.3760/cma.j.cn501225-20240617-00236

Abstract (original English)

Objective: To explore the effect and mechanism of adipose-derived mesenchymal stem cells (ADSCs) on full-thickness skin defects in diabetic rats using proteomics analysis. Methods: This study was a self-control design experimental study. Four 8 to 10 weeks old male Sprague-Dawley rats were selected, and ADSCs were extracted from their epididymal adipose tissue and successfully identified. The third passage of ADSCs were used for the following experiments. Twenty-four 4 to 6 weeks old male Sprague-Dawley rats were selected and the type 2 diabetes model was successfully established. Among them, 16 diabetic rats weighing 350 to 400 g were chosen, and a full-thickness skin defect wound was created on each side of the spine on their backs at the same level. Using the random number table method, the two wounds of each rat were included in experimental group and control group (with 16 wounds in each group), and the cell suspensions containing ADSCs and phosphate buffered saline were injected at multiple points around and at the base of the wounds immediately after injury, respectively. The wound healing rates were calculated at day 7, 10, and 14 after injury in rats. At day 7 after injury in rats, wound tissue was collected and proteins were extracted. Four-dimensional data-independent acquisition label-free quantitative proteomics technology was used for quantitative proteomics analy

What this study does not prove

• This study does not prove SVF is an approved treatment or a replacement for standard care.
• This is preclinical work; animal or laboratory results cannot be applied to humans.

Evidence level

Evidence comes from animal or laboratory studies and has not been confirmed in humans.

How we grade evidence

AnimalsMaleRats, Sprague-DawleyWound HealingProteomicsRatsDiabetes Mellitus, ExperimentalMesenchymal Stem CellsSkinAdipose Tissue

Back & Spine Systemic / IV Type 2 Diabetes Chronic Wound

View on PubMed DOI

[Proteomics analysis of the effect and mechanism of ADSCs on full-thickness skin defects in diabetic rats].

Abstract (original English)

What this study does not prove

Evidence level

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