Pulmonary passage of canine adipose tissue-derived mesenchymal stem cells through intravenous transplantation in mouse model.

Evidence from laboratory and animal studies provides groundwork for understanding mechanisms and potential before human studies continue.

• Level A · Stronger Clinical Evidence
• Level B · Emerging clinical evidence with positive signals
• Level C · Early human research exploring benefits
• Level D · Scientific groundwork from lab and animal studies
• Emerging · Emerging topic under active research

Abstract (original English)

Importance The intravenous administration of adipose tissue-derived mesenchymal stem cells (AdMSCs) in veterinary medicine is an attractive treatment option. On the other hand, it can result in severe complications, including pulmonary thromboembolism (PTE). Objective The present study assessed the occurrence of PTE after the intravenous infusion of canine AdMSCs (cAdMSCs) into experimental animals. Methods Five-week-old male BALB/c hairless mice were categorized into groups labeled A to G. In the control group (A), fluorescently stained 2 × 10 6 cAdMSCs were diluted in 200 μL of suspension and injected into the tail vein as a single bolus. The remaining groups included the following: group B with 5 × 10 6 cells, group C with 3 × 10 6 cells, group D with 1 × 10 6 cells, group E with 1 × 10 6 cells injected twice with a one-day interval, group F with 2 × 10 6 cells in 100 μL of suspension, and group G with 2 × 10 6 cells in 300 μL of suspension. Results Group D achieved a 100% survival rate, while none of the subjects in groups B and C survived ( p = 0.002). Blood tests revealed a tendency for the D-dimer levels to increase as the cell dose increased ( p = 0.006). The platelet count was higher in the low cell concentration groups and lower in the high cell concentration groups ( p = 0.028). A histological examination revealed PTE in most deceased subjects (96.30%). Conclusions a

Evidence level

Evidence from laboratory and animal studies provides groundwork for understanding mechanisms and potential before human studies continue.

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