Redefining 3D cell culture: Human methacryloyl platelet lysates hydrogels for reliable, consistent, and ethical applications.

Evidence from laboratory and animal studies provides groundwork for understanding mechanisms and potential before human studies continue.

• Level A · Stronger Clinical Evidence
• Level B · Emerging clinical evidence with positive signals
• Level C · Early human research exploring benefits
• Level D · Scientific groundwork from lab and animal studies
• Emerging · Emerging topic under active research

Abstract (original English)

The transition to human-derived biomaterials is critical for advancing ethical and clinically relevant three-dimensional (3D) cell culture systems. In this study, we evaluate the performance of human methacryloyl platelet lysates (hPLMA), a xeno-free, human-derived hydrogel, benchmarking it against Matrigel, the gold standard in the field, and a widely used but animal-derived matrix with an unethical tumor origin. Human adipose-derived stem cells (hASCs) were encapsulated in both materials and cultured for 14 days. Both materials support high viability and proliferation for 7 days. However, hPLMA promotes consistent cell growth and intricate networks, while Matrigel induces rapid spreading, leading to massive cell clusters and ultimately the degradation of the hydrogel after 7 days. Although macrophage culture in both materials show low cytokine levels, the transcriptomic profile of hASCs in Matrigel reveal a constant high expression of immune-related genes, especially after 5 days. In contrast, hASCs in hPLMA have lower expression of immune response genes and higher expression of genes associated with cell migration, adhesion, and matrix organization, showing hPLMA's ability to mimic the natural cell environment. These results position hPLMA as a robust, xeno-free platform not only for 3D cell culture applications such as drug screening, organ-on-chip and tissue models, but al

Evidence level

Evidence from laboratory and animal studies provides groundwork for understanding mechanisms and potential before human studies continue.

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