Regenerative potential of muse cells in ROS-mediated cardiac injury: An in vitro oxygen-glucose deprivation/reoxygenation model.

Evidence from laboratory and animal studies provides groundwork for understanding mechanisms and potential before human studies continue.

• Level A · Stronger Clinical Evidence
• Level B · Emerging clinical evidence with positive signals
• Level C · Early human research exploring benefits
• Level D · Scientific groundwork from lab and animal studies
• Emerging · Emerging topic under active research

Abstract (original English)

Myocardial Ischemia-reperfusion injury produces excessive reactive oxygen species, leading to myocardial cell death and debilitated cardiac function. The regenerative properties of cardiac stem/progenitor cells, particularly the Sca-1 + population, remain narrow under ischemia-reperfusion injury conditions. Multilineage stress-enduring cells known as Muse cells, a distinguished subpopulation of SSEA-3 + mesenchymal stromal cells (MSCs), have shown promise in tissue repair due to their stress-enduring, non-tumorigenic, and pluripotent-like properties. This study questioned the regenerative potential of Muse cells in rescuing Sca-1 + cardiac stem cells exposed in vitro to oxygen-glucose deprivation/reoxygenation injury. Muse cells were first isolated from human adipose mesenchymal stromal cells using Magnetic-activated cell sorting for SSEA-3 + cells. Co-culture experiments were conducted to assess the impacts of Muse cells on Sca-1 + cardiac stem cells proliferation, apoptosis, oxidative stress. Spontaneous Cardiac differentiation of Muse cells was assessed using expression of cardiac markers (GATA-4, Myosin light chain 2, Connexin-43, Troponin C1, and Myosin heavy chain 6. Muse cell co-culture with cells exposed to oxygen-glucose deprivation/reoxygenation injury significantly improved the survival and proliferation of Sca-1 + cardiac stem cells, while reducing apoptosis and oxi

Evidence level

Evidence from laboratory and animal studies provides groundwork for understanding mechanisms and potential before human studies continue.

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