Rg1-preconditioned adipose-derived mesenchymal stromal cells alleviate colitis via exosome-mediated Inhibition of macrophage glycolysis through RAS signaling.

Evidence from laboratory and animal studies provides groundwork for understanding mechanisms and potential before human studies continue.

• Level A · Stronger Clinical Evidence
• Level B · Emerging clinical evidence with positive signals
• Level C · Early human research exploring benefits
• Level D · Scientific groundwork from lab and animal studies
• Emerging · Emerging topic under active research

Abstract (original English)

Aims The therapeutic potential of adipose-derived mesenchymal stromal cells (ADSCs) in inflammatory bowel disease (IBD) is well established, yet the mechanisms underlying their immunoregulatory effects remain unclear. Ginsenoside Rg1 has been shown to enhance the immunomodulatory properties of ADSCs. Given the key role of macrophages in IBD pathogenesis, this study aimed to explore whether mesenchymal stromal cells (MSCs), particularly following Rg1 pretreatment, alleviate colitis by modulating macrophage immunometabolism. Main methods In vitro experiments were performed in RAW264.7 macrophages and NCM460 cell. In vivo experiments were performed in C57BL/6 mice. Key findings Rg1 pretreatment upregulated stemness-related genes and downregulated immunogenic markers in ADSCs. In vivo, Rg1-preconditioned ADSCs significantly alleviated DSS-induced colitis and inhibited M1 macrophage polarization by reducing glycolytic activity. Mechanistically, ADSC-derived exosomes delivered miRNAs that suppressed glycolysis and RAS signaling in macrophages, thereby limiting pro-inflammatory polarization and ameliorating colitis. This miRNA is presumed to be miR-574-3p. Significance MSCs alleviate experimental colitis by modulating macrophage immunometabolism, and Rg1 pretreatment further enhances this therapeutic potential. This effect may involve exosomal miRNAs that inhibit macrophage glycolysis

Evidence level

Evidence from laboratory and animal studies provides groundwork for understanding mechanisms and potential before human studies continue.

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