Synergistic effects of mesenchymal stem cell secretome and Wnt10b on hair follicle regeneration in a 3D bioengineering model: a preclinical study

Early human evidence such as case series or small samples is exploring possible benefits.

• Level A · Stronger Clinical Evidence
• Level B · Emerging clinical evidence with positive signals
• Level C · Early human research exploring benefits
• Level D · Scientific groundwork from lab and animal studies
• Emerging · Emerging topic under active research

Abstract (original English)

Background Despite all advances, constructing functional hair follicles remains a significant clinical challenge. Building on recent advancements in mesenchymal stem cells secretome and Wnt signaling, we aimed to fabricate hair follicles in a 3D hydrogel scaffold and assess the effects of placenta-derived MSCs (PMSC) secretome and Wnt10b on follicle formation in ex vivo and in vivo models. Methods A gelatin methacryloyl (GelMA) hydrogel scaffold was engineered via a 3D-printed We engineered a gelatin methacryloyl (GelMA) hydrogel scaffold using a 3D-printed polydimethylsiloxane mold to create a co-culture system for hair follicle biofabrication. Dermal papilla cells (DPCs) and epithelial cells from a donor were isolated and seeded into the scaffold to form microfollicles. We treated hair microfollicles with PMSC secretome, Wnt10b protein, or combination of both. The generated hair microfollicles were assessed using specific molecular and protein markers and then implanted into nude mice skin to investigate the hair growth in vivo. Results After three weeks of co-culturing, the group receiving both secretome and Wnt10b showed 90% cell aggregation and highest hair follicle formation rate at 28% (p Conclusion Our study demonstrates that the combination of PMSC-secretome and Wnt10b in a GelMA hydrogel scaffold effectively promotes hair follicle biofabrication and successful hair fo

Evidence level

Early human evidence such as case series or small samples is exploring possible benefits.

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