SVF
Level D· Preclinical EvidenceAnimal Study

Temporal Gene Expression Changes in Rotator Cuff Tendon Injury and Repair.

Lee ES., Choi JH., Lee YN., Hwang SR., Shim IK., Koh KH.

Animal Study on Tendon Injury, Rotator Cuff, Chronic Wound, Scar, published in J Orthop Res (2026) — summary generated from the PubMed abstract.

This page is generated from the PubMed record. The Thai description is an automated summary of bibliographic fields and the abstract, not a full translation, and is not medical advice.

Study type
Animal Study
Journal
J Orthop Res (2026)
Country
United States
Reported sample size
—
PMID
41456954
DOI
10.1002/jor.70125

Abstract (original English)

Rotator cuff tendon injuries are common, particularly among the elderly. However, promising biomarkers to assess tendon degeneration and healing remain limited. This study aimed to identify dynamic biomarkers by analyzing temporal gene expression and histological changes in a chronic rotator cuff tear model. Using Sprague-Dawley rats, a chronic injury was induced by detaching the supraspinatus tendon and inserting a silastic drain to inhibit spontaneous healing. Tendon samples were collected at multiple time points after injury and after repair for gene expression profiling and histological analysis. Human supraspinatus tendon samples (normal and degenerative) were acquired for validation. A three-dimensional in vitro tendon construct model using human adipose-derived stem cells was subjected to mechanical loading to investigate gene expression under controlled conditions. Tendon-specific markers, including Scleraxis, Tenascin C, and Collagen III, were downregulated early after injury but demonstrated partial recovery with scar formation, limiting their utility as healing indicators. Conversely, Collagen I and YAP1 showed significant downregulation during progressive tendon degeneration and marked upregulation following surgical repair. Histological improvements in collagen organization corresponded with YAP1 re-expression during the recovery phase. YAP1 was consistently reduce

What this study does not prove

  • • This study does not prove SVF is an approved treatment or a replacement for standard care.
  • • This is preclinical work; animal or laboratory results cannot be applied to humans.

Evidence level

Evidence comes from animal or laboratory studies and has not been confirmed in humans.

How we grade evidence
Rotator Cuff InjuriesRats, Sprague-DawleyAnimalsHumansMaleRotator CuffYAP-Signaling ProteinsRatsFemaleMiddle Aged
ShoulderTendon InjuryRotator CuffChronic WoundScar
View on PubMedDOI

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