Tissue regenerative potential of human dental pulp mesenchymal stem cell conditioned medium in wound healing of the medicinal leech

Evidence from laboratory and animal studies provides groundwork for understanding mechanisms and potential before human studies continue.

• Level A · Stronger Clinical Evidence
• Level B · Emerging clinical evidence with positive signals
• Level C · Early human research exploring benefits
• Level D · Scientific groundwork from lab and animal studies
• Emerging · Emerging topic under active research

Abstract (original English)

Background As life expectancy increases, so does the incidence of age-related disorders, including vascular diseases and chronic wounds. These conditions are often associated with impaired angiogenesis and tissue repair, posing significant clinical challenges due to prolonged healing times. Recent studies have highlighted the tissue regenerative potential of the human Mesenchymal Stem Cell-Conditioned Medium (MSC-CM). Building on this, we investigated the ability of CM derived from human Dental Pulp Stem Cells (hDPSC-CM) to support angiogenesis and tissue repair in a surgically injured medicinal leech model. The medicinal leech Hirudo verbana offers a valuable in vivo tool for studying wound healing and regeneration, due to its well-characterized anatomy, regenerative capabilities, and the evolutionary conservation of key immune and vascular pathways. Methods Adult leeches were surgically injured and treated with either FBS-free medium (CTRL) or hDPSC-CM. Animals were sacrificed at 48 h, 72 h, and 1 week post-treatment to evaluate tissue regeneration across experimental groups. Morphological, immunohistochemical, and molecular analyses were conducted. Results hDPSC-CM significantly accelerated wound healing, promoting extracellular matrix remodelling and muscle regeneration. The expression of key markers acid phosphatase, CD31, VEGF, Desmin, AIF-1, and COL Iα1, along with vesse

Evidence level

Evidence from laboratory and animal studies provides groundwork for understanding mechanisms and potential before human studies continue.

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