Tri-Layered Full-Thickness Artificial Skin Incorporating Adipose-Derived Stromal Vascular Fraction Cells, Keratinocytes, and a Basement Membrane.

Evidence from laboratory and animal studies provides groundwork for understanding mechanisms and potential before human studies continue.

• Level A · Stronger Clinical Evidence
• Level B · Emerging clinical evidence with positive signals
• Level C · Early human research exploring benefits
• Level D · Scientific groundwork from lab and animal studies
• Emerging · Emerging topic under active research

Abstract (original English)

Tissue-engineered artificial skin has the potential to enhance wound healing without necessitating extensive surgical procedures or causing donor-site morbidity. The purpose of this study was to examine the possibility of developing tri-layered tissue-engineered full-thickness artificial skin with a basement membrane for clinical use to accelerate wound healing. We engineered full-thickness artificial skin with a basement membrane for wound healing by employing stromal vascular fraction (SVF) cells for the dermal layer and autologous keratinocytes for the epidermal layer. The fabrication of a basement membrane involved the use of 100% bovine collagen and 4% elastin produced through a low-temperature three-dimensional printer. Scaffolds for cells were printed with 100% bovine collagen. The basement membrane underwent evaluations for collagenase degradation, tensile strength, and structural characteristics using scanning electron microscopy. The final tri-layered full-thickness artificial skin included two cell scaffolds with a basement membrane between them. The basement membrane may support cellular attachment without inducing significant cytotoxic effects. This study presents a novel strategy for full-thickness artificial skin development, combining SVF and keratinocytes with an optimized collagen-elastin basement membrane. This method may overcome the significant limitations

Evidence level

Evidence from laboratory and animal studies provides groundwork for understanding mechanisms and potential before human studies continue.

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