Tuning Secretomes for Regenerative Medicine

Evidence from laboratory and animal studies provides groundwork for understanding mechanisms and potential before human studies continue.

• Level A · Stronger Clinical Evidence
• Level B · Emerging clinical evidence with positive signals
• Level C · Early human research exploring benefits
• Level D · Scientific groundwork from lab and animal studies
• Emerging · Emerging topic under active research

Abstract (original English)

In this narrative review, the systematic tailoring of cell-derived secretomes for regenerative medicine is comprehensively discussed. The review begins by evaluating a diverse array of cell sources, including platelets, endothelial cells, osteoblasts, chondrocytes, tenocytes, and mesenchymal stem cells (MSCs). From this foundation, we critically analyze how modulating cell culture conditions can be used to directly influence the therapeutic and paracrine profiles of the resulting secretomes. Specifically, we address the impact of the following critical parameters on secretome fabrication: (i) Culture Medium Supplementation: The use of defined media, supplements, and chemical priming agents. (ii) Cell Cultivation Formats: A comparative analysis of 2D monolayer cultures versus 3D spheroids. (iii) Culture Duration and Fluidics: The effects of culture time alongside static versus dynamic culture systems. (iv) Microenvironmental Stress: The influence of varying oxygen levels (for example, hypoxia) and induced oxidative stress on secretory output.

Evidence level

Evidence from laboratory and animal studies provides groundwork for understanding mechanisms and potential before human studies continue.

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