Wound healing improvement by a multicomponent wound dressing of keratinocyte-imprinted polydimethylsiloxane substrate in a rabbit model.

Evidence from laboratory and animal studies provides groundwork for understanding mechanisms and potential before human studies continue.

• Level A · Stronger Clinical Evidence
• Level B · Emerging clinical evidence with positive signals
• Level C · Early human research exploring benefits
• Level D · Scientific groundwork from lab and animal studies
• Emerging · Emerging topic under active research

Abstract (original English)

Wound healing is a major clinical challenge and regenerative medicine and tissue engineering suggest new ways to overcome such problems. The cell imprinting method can provide cues that regulate cell function or modulate stem cell differentiation into different lineages. In this study, the rabbit keratinocyte-imprinted polydimethylsiloxane (PDMS) substrate (KiPDMS) was made and associated with adipose-derived stem cells (ADSCs), keratinocytes, and collagen scaffold, provided various treatments. After evaluating ADSCs' differentiation into keratinocytes by qPCR and immunocytochemistry techniques, different treatments were implanted in a rabbit ear, histopathological analysis was performed on measuring the epidermal thickness and neovascularization. The histopathological analysis revealed that treated groups increased epidermal thickness (~ 139% up to ~ 998%) and neovascularization (vessel count /mm 2 , ~ 111% to ~ 301%) compared to the control group. Collagen scaffolds stimulated re-epithelialization, and implanting ADSCs with or without other factors enhanced its effect. Adding KiPDMS significantly improved re-epithelialization and neovascularization, especially in combination with differentiated keratinocytes. The complete treatment, consisting of all cellular and non-cellular stimulating factors, associated with a significant improvements in re-epithelialization and neovascul

Evidence level

Evidence from laboratory and animal studies provides groundwork for understanding mechanisms and potential before human studies continue.

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