Yoda1-activated Piezo1 enhances mitophagy in human gingival fibroblasts to promote maxillofacial wound repair

Evidence from laboratory and animal studies provides groundwork for understanding mechanisms and potential before human studies continue.

• Level A · Stronger Clinical Evidence
• Level B · Emerging clinical evidence with positive signals
• Level C · Early human research exploring benefits
• Level D · Scientific groundwork from lab and animal studies
• Emerging · Emerging topic under active research

Abstract (original English)

Background Delayed healing of maxillofacial soft tissue wounds is closely associated with inffammatory injury and mitochondrial dysfunction in fibroblasts, while effective therapeutic strategies remain limited. This study investigated whether activation of the mechanosensitive ion channel Piezo1 by Yoda1 could promote wound healing. Methods An LPS-induced inflammatory model was established in human gingival fibroblasts (HGFs) and treated with Yoda1. Intracellular calcium influx, cell viability, oxidative stress, mitochondrial membrane potential, apoptosis, migration, wound closure, and type I collagen expression were assessed. The involvement of the PINK1/Parkin-mediated mitophagy pathway was analyzed. An in vivo inflammatory wound model was used to evaluate therapeutic efficacy. Results Yoda1 significantly increased intracellular calcium influx, improved cell viability, reduced oxidative stress, restored mitochondrial membrane potential, and inhibited apoptosis in LPS-treated HGFs. It also enhanced HGFs migration, wound closure, and type I collagen expression in vitro . Mechanistically, these effects were associated with activation of the PINK1/Parkin-mediated mitophagy pathway. In vivo , Yoda1 accelerated wound closure, accompanied by increased collagen deposition and improved tissue regeneration. Conclusion Activation of Piezo1 by Yoda1 can alleviate its inflammatory damage

Evidence level

Evidence from laboratory and animal studies provides groundwork for understanding mechanisms and potential before human studies continue.

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