AB040. Early and long-term protective effect of uncultured adipose stromal vascular fraction against renal ischemia-reperfusion injury via pre-ischemic administration

Evidence from laboratory and animal studies provides groundwork for understanding mechanisms and potential before human studies continue.

• Level A · Stronger Clinical Evidence
• Level B · Emerging clinical evidence with positive signals
• Level C · Early human research exploring benefits
• Level D · Scientific groundwork from lab and animal studies
• Emerging · Emerging topic under active research

Abstract (original English)

Background Ischemia-reperfusion (IR) induced acute kidney injury (AKI) is the common clinical syndrome. Stem/progenitor cells therapy is a promising option to foster the intrinsic capacity of renal regeneration. However, several challenges still remain due to the potential risks during in vitro cell culture, low retention rate after transplantation and unclear effect on the progression of chronic kidney disease (CKD). Adipose stromal vascular fraction (SVF) is regarded as an attractive cell source for cell-based therapy without need of in vitro expansion. Preconditioning with ischemia has been suggested to be a useful method to induce a delay of lethal cell injury, enhance cellular tolerance to injurious effects of IR, and promote cell retention and survival. The purpose of this study is to investigate the effect of pre-administration of uncultured adipose SVF on IR induced AKI and progression of fibrosis. Methods SVF was isolated from rat epididymal adipose tissue and characterized by flow cytometric analysis. Cell proliferation and scrape wound healing assay was performed by a co-culture system to evaluate the effects of SVF pre-hypoxic administration on proliferation, migration and apoptosis of renal tubular epithelial cells in hypoxic environment. IR induced kidney injury model was established and CM-DiI labeled SVF was injected to the subcapsular pre- or post-ischemia. Ren

Evidence level

Evidence from laboratory and animal studies provides groundwork for understanding mechanisms and potential before human studies continue.

Browse all related research

Filter the research library by this study's title keywords, author, or publication year.