Early histological advantage of CD271-positive adipose-derived mesenchymal stromal cells isolated from the infrapatellar fat pad in experimental knee osteoarthritis.

Evidence from laboratory and animal studies provides groundwork for understanding mechanisms and potential before human studies continue.

• Level A · Stronger Clinical Evidence
• Level B · Emerging clinical evidence with positive signals
• Level C · Early human research exploring benefits
• Level D · Scientific groundwork from lab and animal studies
• Emerging · Emerging topic under active research

Abstract (original English)

Background Mesenchymal stromal cells (MSCs) have been increasingly explored as a minimally invasive treatment for osteoarthritis (OA), primarily due to their anti-inflammatory, immunomodulatory, and analgesic properties. However, MSC populations are biologically heterogeneous, and optimal cell phenotypes for intra-articular therapy remain unclear. CD271 has been identified as a marker associated with MSC subpopulations with enhanced regenerative potential. The aim of this study is to evaluate whether intra-articular injection of phenotype-selected CD271-positive adipose-derived MSCs (AD-MSCs) provides additional structural benefits compared with unselected AD-MSCs in experimental OA. Methods OA was induced in athymic rats by intra-articular injection of monoiodoacetate. Human infrapatellar fat pad-derived AD-MSCs were isolated and separated into CD271-positive fractions using magnetic-activated cell sorting. One week after OA induction, animals received intra-articular injection of saline, plastic-adherent AD-MSCs (PA-AD-MSCs), or CD271-positive AD-MSCs. Cartilage degeneration was assessed macroscopically and histologically using the Histologic/Histochemical Grading System (HHGS). Pain-related neuropeptides in dorsal root ganglia were analyzed by immunohistochemistry, and synovial inflammatory cytokines were quantified by ELISA. Results Both MSC-treated groups showed significan

Evidence level

Evidence from laboratory and animal studies provides groundwork for understanding mechanisms and potential before human studies continue.

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