Level D· Preclinical EvidenceAnimal Study

Evaluation of an AD-MSC Supernatant-Loaded Thermosensitive Hydrogel for Cartilage Protection in Osteoarthritis.

Zhang J., Zhang S., Cheng M., Han Y., Zhang H., Xue H.

Animal Study on Knee Osteoarthritis, Osteoarthritis, Cartilage Damage, Chronic Inflammation, published in Int J Mol Sci (2026) — summary generated from the PubMed abstract.

This page is generated from the PubMed record. The Thai description is an automated summary of bibliographic fields and the abstract, not a full translation, and is not medical advice.

Study type: Animal Study
Journal: Int J Mol Sci (2026)
Country: Switzerland
Reported sample size: —
PMID: 41828622
DOI: 10.3390/ijms27052405

Abstract (original English)

Knee osteoarthritis (KOA) is a degenerative joint disorder characterized by chronic inflammation and progressive cartilage degradation. Mesenchymal stem cell (MSC)-based therapies have demonstrated therapeutic potential; however, increasing evidence suggests that their efficacy primarily arises from paracrine factors, highlighting the potential of cell free approaches. In this study, we developed an injectable, thermosensitive composite hydrogel incorporating adipose-derived MSC (AD-MSC) supernatant within a Pluronic F-127 (PF-127)/sodium hyaluronate (HA) matrix. The hydrogel exhibited a solution state at a low temperature and rapidly transitioned into a stable gel at a physiological temperature without chemical crosslinkers. Microstructural analysis revealed a porous, interconnected three-dimensional network favorable for the sustained release of bioactive factors. In a rat model of KOA, intra-articular administration of the AD-MSC supernatant-loaded hydrogel significantly improved joint architecture and locomotor performance, alleviated synovial inflammation, and preserved cartilage integrity. Radiographic and histological assessments demonstrated reduced cartilage degeneration and subchondral bone alterations. Moreover, the treatment markedly decreased intra-articular levels of proinflammatory cytokines (IL-1β and TNF-α) and the cartilage degradation marker CTX-II in a time-

What this study does not prove

• This study does not prove SVF is an approved treatment or a replacement for standard care.
• This is preclinical work; animal or laboratory results cannot be applied to humans.

Evidence level

Evidence comes from animal or laboratory studies and has not been confirmed in humans.

How we grade evidence

AnimalsMesenchymal Stem CellsRatsHydrogelsAdipose TissueCartilage, ArticularOsteoarthritis, KneeHyaluronic AcidMalePoloxamer

Knee Knee Osteoarthritis Osteoarthritis Cartilage Damage Chronic Inflammation

View on PubMed DOI

Evaluation of an AD-MSC Supernatant-Loaded Thermosensitive Hydrogel for Cartilage Protection in Osteoarthritis.

Abstract (original English)

What this study does not prove

Evidence level

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