Hypoxic preconditioning drives metabolic reprogramming to increase the therapeutic efficacy of adipose mesenchymal stem cells in diabetic wounds.

Evidence from laboratory and animal studies provides groundwork for understanding mechanisms and potential before human studies continue.

• Level A · Stronger Clinical Evidence
• Level B · Emerging clinical evidence with positive signals
• Level C · Early human research exploring benefits
• Level D · Scientific groundwork from lab and animal studies
• Emerging · Emerging topic under active research

Abstract (original English)

Background Hypoxic pretreatment improves the efficacy of adipose-derived mesenchymal stem cells (ADSCs) in the repair of chronic refractory wounds; however, the underlying molecular mechanisms remain poorly understood. Energy metabolism serves as a crucial regulatory factor that influences stem cell fate, proliferation, and self-renewal capabilities. In this study, we established a diabetic skin wound model to systematically investigate the effects of the diabetic microenvironment and hypoxic pretreatment on the energy metabolism and functions of ADSCs, aiming to elucidate the role of adaptive metabolic regulation in increasing stem cell survival rates following transplantation. Methods An in vitro diabetic cell model was established to systematically evaluate the effects of high glucose and hypoxic preconditioning on the proliferation, migration, apoptosis, and cytokine secretion of ADSCs via PCR analysis and CCK-8, cell scratch, and apoptosis assays. The impact of high glucose and hypoxic preconditioning on the energy metabolism characteristics of ADSCs was investigated with a Seahorse XF Analyzer. The regulatory effects of the mTOR/HIF-1α signalling pathway on the energy metabolism and cellular functions of ADSCs was investigated via mTOR pathway inhibitors and siHIF transfection. The therapeutic efficacy of hypoxia preconditioned ADSCs in diabetic wound healing was determin

Evidence level

Evidence from laboratory and animal studies provides groundwork for understanding mechanisms and potential before human studies continue.

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