Overexpression of IL-10 in Adipose Mesenchymal Stem Cells Promotes Wound Healing in Diabetic Mice.

Evidence from laboratory and animal studies provides groundwork for understanding mechanisms and potential before human studies continue.

• Level A · Stronger Clinical Evidence
• Level B · Emerging clinical evidence with positive signals
• Level C · Early human research exploring benefits
• Level D · Scientific groundwork from lab and animal studies
• Emerging · Emerging topic under active research

Abstract (original English)

Abstract Background: Diabetic ulcer is a serious chronic non-healing wound, which often leads to amputation or even death, causing great damage to the patients and their families. In recent years, stem cells are increasingly studied for tissue repair. Adipose tissue-derived mesenchymal stem cells (Adipose-derived mesenchymal stem cells, ADSCs) have the advantages of wide source and easy access. ADSCs can accelerate wound healing and reduce scar hyperplasia, especially for chronic wounds, patients cannot heal with traditional treatments, and ADSCs bring hope to these patients. Our study intends to overexpress IL-L 10 in ADSCs by genetic engineering technology, transplant ADSC-IL 10 to diabetic mouse wounds, and use the role of IL-10 in promoting M2 macrophages transformation, combined with ADSCs to achieve rapid healing of diabetic ulcer wounds. Methods : The expression of IL-10 protein in the supernatant of ADSC-IL10 was assessed using an ELISA kit. Flow cytometry was employed to analyze the surface stem cell markers (CD44, CD73, CD90, CD105) of ADSC-IL10. Additionally, the migration and proliferation of ADSC-IL10 were evaluated through cell scratch assays and MTT assays. The lipogenic marker PPARγ and the osteogenic marker RUNX2 were detected using fluorescent real-time quantitative PCR. Conditioned media from ADSC-IL10 (ADSC-IL10-CM) and ADSC-PCDH (ADSC-CM) were employed to c

Evidence level

Evidence from laboratory and animal studies provides groundwork for understanding mechanisms and potential before human studies continue.

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