SVF
Level D· Scientific groundwork from lab and animal studiesAnimal StudyPubMed

Serum C-peptide level correlates with the course of muscle tissue healing in the rabbit model of critical limb ischemia.

Tauber Z., Cizkova K., Janikova M., Jurcikova J., Vitkova K., Pavliska L.

Animal Study on Diabetic Foot, Peripheral Artery Disease, Chronic Wound, Scar, published in Biomed Pap Med Fac Univ Palacky Olomouc Czech Repub (2018) — summary generated from the PubMed abstract.

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Level D· Scientific groundwork from lab and animal studiesEvidence level of this study

Evidence from laboratory and animal studies provides groundwork for understanding mechanisms and potential before human studies continue.

  • Level A · Stronger Clinical Evidence
  • Level B · Emerging clinical evidence with positive signals
  • Level C · Early human research exploring benefits
  • Level D · Scientific groundwork from lab and animal studies
  • Emerging · Emerging topic under active research
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This page is generated from the PubMed record. The Thai description is an automated summary of bibliographic fields and the abstract, not a full translation, and is not medical advice.

Study type
Animal Study
Journal
Biomed Pap Med Fac Univ Palacky Olomouc Czech Repub (2018)
Country
Czech Republic
Reported sample size
—
Source database
PubMed
PMID
30198519
DOI
10.5507/bp.2018.048

Abstract (original English)

The therapeutic potential of adipose-derived stem cell conditioned medium (ASC-CM) was studied in the rabbit model of critical limb ischemia (CLI). Rabbits received treatment with ASC-CM or placebo. Gastrocnemius muscle tissue was collected 35 days after ischemia induction. Ischemic changes were evaluated in hematoxylin-eosin stained tissues for early (necrotic lesions/granulation tissue) and late (fibrous scars) phases of tissue repair. The expression of proangiogenic miR-126 was also evaluated using in situ hybridization. The levels of cytokines, insulin, and C-peptide were measured in blood. Early repair phases were observed more often in placebo-treated samples (45.5%) than in ASC-CM-treated ones (22.2%). However, the difference was not statistically significant. We demonstrated a statistically significant positive correlation between the early healing phases in tissue samples and C-peptide levels in peripheral blood. The expression of proangiogenic miR-126 was also shown in a number of structures in all phases of ischemic tissue healing. Based on our results, we believe that treatment with ASC-CM has the potential to accelerate the healing process in ischemic tissues in the rabbit model of CLI. The whole healing process was accompanied by miR-126 tissue expression. C-peptide could be used to monitor the course of the tissue healing process.

What this study does not prove

  • • This study does not prove SVF is an approved treatment or a replacement for standard care.
  • • This is preclinical work; animal or laboratory results cannot be applied to humans.

Evidence level

Evidence from laboratory and animal studies provides groundwork for understanding mechanisms and potential before human studies continue.

How we grade evidence
AdultAnimalsC-PeptideCicatrixCulture Media, ConditionedCytokinesDiabetes Mellitus, ExperimentalDiabetic FootDisease Models, AnimalFibrosis
Ankle & FootSystemic / IVDiabetic FootPeripheral Artery DiseaseChronic WoundScar
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