SVF
Level D· Preclinical EvidenceAnimal Study

Unveiling the therapeutic role of 3D-cultured mesenchymal stem cells in diabetic foot ulcers through transcriptomic integration and fibroblast modulation.

Öztürk E., Bicer M.

Animal Study on Diabetic Foot, Chronic Wound, Chronic Inflammation, published in Mol Biol Rep (2026) — summary generated from the PubMed abstract.

This page is generated from the PubMed record. The Thai description is an automated summary of bibliographic fields and the abstract, not a full translation, and is not medical advice.

Study type
Animal Study
Journal
Mol Biol Rep (2026)
Country
Netherlands
Reported sample size
—
PMID
41915247
DOI
10.1007/s11033-026-11741-x

Abstract (original English)

BACKGROUND: Diabetic foot ulcers (DFUs) are among the most severe complications of diabetes mellitus and remain difficult to manage due to chronic inflammation, defective angiogenesis, delayed tissue repair, which increase the risk of recurrence and limb amputation. Standard treatments, such as debridement, infection management, pressure off-loading and revascularization, are commonly used, however; these interventions often inadequate to fully restore effective wound repair. Mesenchymal stem cells (MSCs) have attracted remarkable interest due to their potential regenerative ability and paracrine activity. Nevertheless, the molecular interaction between MSCs and fibroblasts under hyperglycemic conditions has not been fully elucidated. OBJECTIVE: This study aimed to examine differentially expressed genes (DEGs) associated with DFUs and MSC-related regenerative mechanisms using transcriptomic datasets (such as GSE143735, GSE199939, and GSE217709). METHODS AND RESULTS: Differentially expressed genes and protein-protein interaction (PPI) network analysis were performed to determine central regulatory genes. Four key genes including CXCL1, MMP9, THBS1, and POSTN were recognized as hub genes related to inflammatory response, extracellular matrix reorganization, and angiogenesis. For experimental validation, L929 murine fibroblasts were exposed to high-glucose conditions to set-up an

What this study does not prove

  • • This study does not prove SVF is an approved treatment or a replacement for standard care.
  • • This is preclinical work; animal or laboratory results cannot be applied to humans.

Evidence level

Evidence comes from animal or laboratory studies and has not been confirmed in humans.

How we grade evidence
Mesenchymal Stem CellsAnimalsFibroblastsMicePeriostinWound HealingTranscriptomeDiabetic FootThrombospondin 1Chemokine CXCL1
Ankle & FootSystemic / IVDiabetic FootChronic WoundChronic Inflammation
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